Maltose Binding Protein Antikörper

Produktnummer ABIN3020578

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Maltose Binding Protein in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Maltose Binding Protein Antikörper (ABIN3020578)

Target: Maltose Binding Protein (MBP)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Maltose Binding Protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Maltose Binding Protein Antikörper

Aufreinigung: Affinity purification

Immunogen: An MBP-Tag fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000 1:3000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C / -80 °C

Referenzen für anti-Maltose Binding Protein Antikörper (ABIN3020578)

Xin, Zhang, Zhang, Li, Wang, Wang, Wang, Qiu, Song: "CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules." in: Developmental and comparative immunology, Vol. 53, Issue 2, pp. 339-48, (2016) (PubMed).

Details zu Maltose Binding Protein

Target: Maltose Binding Protein (MBP)

Andere Bezeichnung: Maltose Binding Protein

Hintergrund: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (myelin basic protein) TAG in some expression systems.